rabbit polyclonal anti syt1  (Proteintech)

Journal: Frontiers in Behavioral Neuroscience

Article Title: Altered cognition and anxiety in adolescent offspring whose mothers underwent different-pattern maternal sleep deprivation, and cognition link to hippocampal expressions of Bdnf and Syt-1

doi: 10.3389/fnbeh.2022.1066725

Figure Lengend Snippet: Effects of MSD on the expression of hippocampal of BDNF and Syt-1 proteins in the offspring. (A) The expression levels of BDNF ( n = 12, 6 females and 6 males in each treatment group) and Syt-1 ( n = 12, 6 females and 6 males in each treatment group) proteins in the hippocampus at 2 months. The results of protein quantification are depicted in (B,C) . ** P < 0.01 vs. Male CON group; ## P < 0.01 vs. Male CSD3h group; $$ P < 0.01 vs. Male CSD6h group; aa P < 0.01 vs. Female CON group; bb P < 0.01 vs. Female CSD3h group; cc P < 0.01 vs. Female CSD6h group.

Article Snippet: The membrane was subsequently incubated with the rabbit anti-BDNF (1:1000; Abcam, Cambridge, UK) and rabbit anti-Syt-1 (1:1000; Bioss, Beijing, China) primary antibodies, and the horseradish peroxidase (HRP) labeled goat anti-rabbit IgG (Zsbio, ZB-2301) secondary antibody, according to the manufacturer's instructions.

Techniques: Expressing

Journal: Frontiers in Behavioral Neuroscience

Article Title: Altered cognition and anxiety in adolescent offspring whose mothers underwent different-pattern maternal sleep deprivation, and cognition link to hippocampal expressions of Bdnf and Syt-1

doi: 10.3389/fnbeh.2022.1066725

Figure Lengend Snippet: The effects of MSD on the expression of hippocampal of BDNF and Syt-1 mRNA in the offspring. The mRNA expression levels of BDNF ( n = 16, 8 females and 8 males in each treatment group) and Syt1 ( n = 16, 8 females and 8 males in each treatment group) in the hippocampus at 2 months. The results of mRNA quantification are depicted in (A,B) . ** P < 0.01 vs. Male CON group; # P < 0.05 and ## P < 0.01 vs. Male CSD3h group; $ P < 0.05 and $$ P < 0.01 vs. Male CSD6h group; a P < 0.05 and aa P < 0.01 vs. Female CON group; b P < 0.05 and bb P < 0.01 vs. Female CSD3h group; c P < 0.05 and cc P < 0.01 vs. Female CSD6h group.

Article Snippet: The membrane was subsequently incubated with the rabbit anti-BDNF (1:1000; Abcam, Cambridge, UK) and rabbit anti-Syt-1 (1:1000; Bioss, Beijing, China) primary antibodies, and the horseradish peroxidase (HRP) labeled goat anti-rabbit IgG (Zsbio, ZB-2301) secondary antibody, according to the manufacturer's instructions.

Techniques: Expressing

Journal: Cell reports

Article Title: Cytosolic LPS-induced caspase-11 oligomerization and activation is regulated by extended synaptotagmin 1

doi: 10.1016/j.celrep.2023.112726

Figure Lengend Snippet: (A) The cell lysates of LPS-primed WT and ESyt1−/− BMDMs were incubated with biotin-LPS or LPS-primed WT and ESyt1−/− BMDMs were transfected with Biotin-LPS using electroporation. LPS-binding proteins were pulled down by avidin beads and blotted with anti-Casp-11 and anti-E-Syt1 antibodies.

Article Snippet: Rabbit polyclonal anti-E-Syt1 , Invitrogen , Cat# PA5-90448; RRID:AB_2857152.

Techniques: Incubation, Transfection, Electroporation, Binding Assay, Avidin-Biotin Assay

Journal: Cell reports

Article Title: Cytosolic LPS-induced caspase-11 oligomerization and activation is regulated by extended synaptotagmin 1

doi: 10.1016/j.celrep.2023.112726

Figure Lengend Snippet: (A) Immunoblot analysis of E-Syt1 in cell lysates of WT and ESyt1−/− BMDMs primed with LPS (500 ng/mL, 5 h) and treated with DSS.

Article Snippet: Rabbit polyclonal anti-E-Syt1 , Invitrogen , Cat# PA5-90448; RRID:AB_2857152.

Techniques: Western Blot

Journal: Cell reports

Article Title: Cytosolic LPS-induced caspase-11 oligomerization and activation is regulated by extended synaptotagmin 1

doi: 10.1016/j.celrep.2023.112726

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-E-Syt1 , Invitrogen , Cat# PA5-90448; RRID:AB_2857152.

Techniques: Virus, Recombinant, Transfection, Enzyme-linked Immunosorbent Assay, Software

Journal: iScience

Article Title: Synaptotagmin 2 is ectopically overexpressed in excitatory presynapses of a widely used CaMKΙΙα-Cre mouse line

doi: 10.1016/j.isci.2022.104692

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal antibody anti-Syt1 , Synaptic Systems , Cat#105103; RRID: AB_11042457.

Techniques: Virus, Recombinant, cDNA Synthesis, Western Blot, Stripping, Immunodetection, Plasmid Preparation, Isolation, Sequencing, Software

Journal: Molecular & cellular proteomics : MCP

Article Title: Phosphoproteome Analysis Identifies a Synaptotagmin-1-Associated Complex Involved in Ischemic Neuron Injury.

doi: 10.1016/j.mcpro.2022.100222

Figure Lengend Snippet: FIG. 3. Immature neurons with low expression of Syt1 exhibit enhanced tolerance to cerebral ischemia in vivo and OGD in vitro. A and B, representative Western blot image (A) and quantitative analysis (B) of the expression level of Syt1 in cultured immature and mature

Article Snippet: The proteins were transferred to nitrocellulose (NC, HATF00010, Millipore) filters at 80 V for 5 h. The NC membrane was initially blocked with 5% nonfat milk and 2% goat serum (16210064, Thermo Fisher(v/v)) in trisbuffered saline with 0.1% Tween 20 (93773, Sigma) at room temperature for 1 h. Monoclonal antibodies to β-actin (HC201-02, TransGen Biotech), polyclonal antibody to Syt1 (CSB-PA019553GA01HU, CUSABIO Biotech), rabbit polyclonal anti-phosphotyrosine (Cat# PTM-702, PTM BIO) were employed for Western blot analyses as primary antibodies at 4 ◦C overnight.

Techniques: Expressing, In Vivo, In Vitro, Western Blot, Cell Culture

Journal: Molecular & cellular proteomics : MCP

Article Title: Phosphoproteome Analysis Identifies a Synaptotagmin-1-Associated Complex Involved in Ischemic Neuron Injury.

doi: 10.1016/j.mcpro.2022.100222

Figure Lengend Snippet: FIG. 4. Administration of the Tat-Syt1T112A peptide protects against neuronal injury in cerebral ischemia. A, schematic diagram of mouse full-length Syt1 and tat peptides for Syt1WT and Syt1T112A. B, representative images of hippocampal neurons incubated with either Tat-V5

Article Snippet: The proteins were transferred to nitrocellulose (NC, HATF00010, Millipore) filters at 80 V for 5 h. The NC membrane was initially blocked with 5% nonfat milk and 2% goat serum (16210064, Thermo Fisher(v/v)) in trisbuffered saline with 0.1% Tween 20 (93773, Sigma) at room temperature for 1 h. Monoclonal antibodies to β-actin (HC201-02, TransGen Biotech), polyclonal antibody to Syt1 (CSB-PA019553GA01HU, CUSABIO Biotech), rabbit polyclonal anti-phosphotyrosine (Cat# PTM-702, PTM BIO) were employed for Western blot analyses as primary antibodies at 4 ◦C overnight.

Techniques: Incubation

Journal: Molecular & cellular proteomics : MCP

Article Title: Phosphoproteome Analysis Identifies a Synaptotagmin-1-Associated Complex Involved in Ischemic Neuron Injury.

doi: 10.1016/j.mcpro.2022.100222

Figure Lengend Snippet: FIG. 5. Kcnq2 interacts with Syt1 and alleviates Syt1-mediated neuronal injury by OGD treatment. A and B, representative LC–MS/MS spectrum of Kcnq2 phosphorylation at the S52 site (A) and quantification of KCNQ phosphorylation at the S52 site and Kcnq2 protein expression level (B) in the hippocampus after sham or acute MCAO ischemia for 2 h. (sham: n = 3 repeat MS, MCAO: n = 3 repeat MS). C and D, representative images (C) and quantitative analysis (D) of the average length of dendrites of cultured hippocampal neurons transfected with pFUGW-GFP and either an empty vector or a plasmid encoding Kcnq2WT, Kcnq2S52A, or Kcnq2S52D at DIV 10 followed by control or OGD treatment for 2 h at DIV 14. The scale bar represents 100 μm and 10 μm (magnified images). Transfected neurons were chosen randomly, and images were acquired using a confocal microscope. (control: vector: n = 35 cells/3 cultures, Kcnq2WT: n = 34 cells/3 cultures, Kcnq2S52A: n =

Article Snippet: The proteins were transferred to nitrocellulose (NC, HATF00010, Millipore) filters at 80 V for 5 h. The NC membrane was initially blocked with 5% nonfat milk and 2% goat serum (16210064, Thermo Fisher(v/v)) in trisbuffered saline with 0.1% Tween 20 (93773, Sigma) at room temperature for 1 h. Monoclonal antibodies to β-actin (HC201-02, TransGen Biotech), polyclonal antibody to Syt1 (CSB-PA019553GA01HU, CUSABIO Biotech), rabbit polyclonal anti-phosphotyrosine (Cat# PTM-702, PTM BIO) were employed for Western blot analyses as primary antibodies at 4 ◦C overnight.

Techniques: Liquid Chromatography with Mass Spectroscopy, Phospho-proteomics, Expressing, Cell Culture, Transfection, Plasmid Preparation, Control, Microscopy

Journal: Molecular & cellular proteomics : MCP

Article Title: Phosphoproteome Analysis Identifies a Synaptotagmin-1-Associated Complex Involved in Ischemic Neuron Injury.

doi: 10.1016/j.mcpro.2022.100222

Figure Lengend Snippet: FIG. 6. Kcnq2 interacts with Anxa6 and alleviates neuronal injury by inhibiting Syt1. A, representative image of the glutathione-S- transferase (GST) pull-down assay of Flag-tagged Kcnq2 (Kcnq2-Flag) to immobilize GST fused to full-length Anxa6 or GST alone followed

Article Snippet: The proteins were transferred to nitrocellulose (NC, HATF00010, Millipore) filters at 80 V for 5 h. The NC membrane was initially blocked with 5% nonfat milk and 2% goat serum (16210064, Thermo Fisher(v/v)) in trisbuffered saline with 0.1% Tween 20 (93773, Sigma) at room temperature for 1 h. Monoclonal antibodies to β-actin (HC201-02, TransGen Biotech), polyclonal antibody to Syt1 (CSB-PA019553GA01HU, CUSABIO Biotech), rabbit polyclonal anti-phosphotyrosine (Cat# PTM-702, PTM BIO) were employed for Western blot analyses as primary antibodies at 4 ◦C overnight.

Techniques: Pull Down Assay

Journal: iScience

Article Title: Real-time three-dimensional tracking of single vesicles reveals abnormal motion and pools of synaptic vesicles in neurons of Huntington's disease mice

doi: 10.1016/j.isci.2021.103181

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal, biotinylated anti-Syt1 , Synaptic Systems , 105 103BT; RRID:AB_2619768.

Techniques: Recombinant, Software